Pessi Margulies acquired her art education at the Hebrew University in Jerusalem and in Tel Aviv with artists Yair Garbuz and Rafi Lavi. She continued her education at the School of Visual Arts and New York University, where she studied art and psychology and received her Masters Diploma in art therapy.
Her work is in numerous private collections and has been shown in Israel and the USA. Pessi resides and works in New York since 1991.